2 edition of Uptake, storage and release of 5-hydroxytryptamine by mast cells in vitro. found in the catalog.
Uptake, storage and release of 5-hydroxytryptamine by mast cells in vitro.
1. Acta Physiol Scand. Aug;79(4) Uptake of 5-hydroxytryptamine by rat peritoneal mast cells in vitro. Jansson SE. PMID: 1. The aggregation of blood platelets was measured in vitro at different time intervals after the addition of 5‐hydroxytryptamine (5‐HT) or of reserpine to platelet‐rich plasma of untreated rabbits and of rabbits injected with reserpine and 5‐HT, i.e. while the platelets were taking up 5‐HT or releasing it under the influence of reserpine.
Uptake and turnover of dopamine (DA) in rat peritoneal mast cells were studied by a cytofluorometric technique. The main advantage of the method is that it permits the study of the distribution of amine content within populations of cells. Catecholamines and indolamines can be differentiated, but subtler structural differences in this group of compounds cannot be distinguished. Stuart A. Slorach and Börje Uvnäs, Dissociation of Histamine Release and 22Na+ Uptake in Rat Mast Cells Exposed to Compound 48/80 in vitro, Acta Physiologica Scandinavica, 76, 1‐2, (), ().
Mast cells isolated from the former group of animals showed a higher degree of histamine release upon challenge in vitro with egg albumin than those from the latter group. Using the lower amount. Reasons for performing study: 5‐hydroxytryptamine (5‐HT; serotonin) is a potent vasoconstrictor of equine digital blood vessels and has been implicated in the pathogenesis of acute laminitis.. Objectives: The aims of this study were firstly to examine whether cells of the digital blood vessel wall exhibited an active uptake mechanism for 5‐HT and to characterise its efficiency; and.
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Gripenberg, Inhibition by Reserpine, Guanethidine and Imipramine of the Uptake of 5‐Hydroxytryptamine by Rat Peritoneal Mast Cells in Vitro, Acta Physiologica Scandinavica, /jtbx, 96, 3, (), ().Cited by: The release of 5‐hydroxytryptamine (5‐HT) and granules from peritoneal mast cells during in vitro and in vivo anaphylaxis and after treatment with compound 48/80 in vitro was investigated by quantitative cytochemical methods.
Sensitization was achieved by s.c. injections of horse serum with H. pertussis, and anaphylaxis was observed 3–30 weeks by: UPTAKE OF AMINES Histamine and 5-hydroxytryptamine, both in vivo and in vitro, are taken up by mast cells of the peritoneal fluid of the rat (Furano and Green, a) and by neoplastic mast cells of the mouse, the latter cells derived from both the Dunn-Potter and the Furth mastocytomas (Day and Green, b; Green, b; Furano and Green, b).Cited by: The release of 5-hydroxytryptamine (5-HT) and histamine from rat peritoneal mast cells was studied.
Major variations of amine content per cell were noted. Mast cells from the peritoneal and pleural cavities of actively sensitized rats were isolated and incubated with biogenic amines (5-hydroxytryptamine and dopamine) with or without pretreatment with specific antigen.
An anaphylactic reaction resulting in the release of % of the histamine in the cells led to a slightly reduced amine by: 2. Uptake, distribution storage and release of 5-hydroxytryptamine by mast cells in vitro.
book turnover of 5-Hydroxytryptamine (5-HT) was studied by cytofluorometric analysis of whole mast cells and individual granules. Injection of 5-HT as well as 5-Hydroxytryptophan (5-HTP) intraperitoneally or subcutaneously resulted in a parallel uptake of 5-HT in cells and granules.
Intraperitoneal injections of 5-HT in such small quantities that may be available under. Uptake, storage and secretion of 5‐hydroxytryptamine and its amino acid precursor by dispersed rat pancreas acinar cells. L Stern.
Search for more papers by this author. Rat pancreas acinar cells contain 5‐hydroxytryptamine (5‐HT; +/‐ ng/i.u. amylase), all of which can be accounted for by the 5‐HT recovered from the. Biosynthesis and Release of Catecholamines Uptake and Storage of Catecholamines Synthesis, Uptake and Binding of Histamine and 5-Hydroxytryptamine in Mast Cells The Binding of Acetylcholine by Brain Particles In Vitro Metabolism of Monoamines by Blood Platelets and Relation to 5-Hydroxytryptamine Liberation.
Uptake of i-hwliowtmptolihan and the loimztum of 5-h~droxvtmptammc 1n expcmmcnt analogow to that shown m tig 2 w zth by di wt` ti N ptophan 3 2 Uptalzc of htstatrte and 5-HT The cells art vitro also took up histamine and 5-HT, uptake depending on the concentration of the amine in the medium (fig 3) An indication of the ability of the cells to.
Publisher Summary. This chapter presents the study of the in vitro release and uptake of noradrenaline in the rat iris. Albino rats of the Sprague–Dawley strain were used. Incubation of the iris with up to μg/ml of noradrenaline (NA) for 2–4 h caused but a slight or no increase in the fluorescence intensity of the adrenergic nerve net, whether, or not 1 mg/ml of glucose and up to 1 mg.
Summary. Light-microscopic autoradiographs of the adrenal medulla at various intervals after the intravenous injection of [3 H] 5-HTP, [3 H] 5-HT, [3 H] noradrenaline and [3 H] adrenaline have been distribution of silver grains following [3 H] 5-HTP uptake was found to be uniform over each of the two main cell populations, adrenaline-storing (A) cells and noradrenaline-storing (NA.
BIOCHEMICAL MEDIC () Uptake and Release of 5-Hydroxytryptamine by Rabbit Platelets II. Kinetic Analysis of Inhibition by Chlorpheniramine Maleate VINCENT J.
CUSIMANO AND D. SIVA SANKARI Division 'to 'Research Laboratories, Queens Children's Psychiatric Center, Bellerose, New York Received July Chlorpheniramine. Human blood platelets were incubated with 5‐hydroxytryptamine and with tryptamine and the uptake of each amine measured.
The uptake of tryptamine, unlike that of 5‐hydroxytryptamine, was linearly related to the concentration of the amine in the surrounding fluid, was similar in amount at 0° and 37° C and varied directly with the p H of the solution.
Mast Cells and 5‐HT: Uptake of labelled 5‐hydroxytryptamine (5‐HT) and 5‐hydroxytryptophan in relation to storage of 5‐HT in individual rat mast cells Martin Ritzén Institute for Medical Cell Research and Genetics, Medical Nobel Institute, Karolinska Institutet, Stockholm, Sweden.
SCHINDLER R, DAY M, FISCHER GA. Culture of neoplastic mast cells and their synthesis of 5-hydroxytryptamine and histamine in vitro. Cancer Res. Jan; 19 (1)– DAY M, GREEN JP. The uptake of amino acids and the synthesis of amines by neoplastic mast cells in culture.
J Physiol. Nov; – [PMC free article]. HISTAMINE is released from mast cells following the injection into animals of histamine liberators. Coincident with the release of histamine other substances have been detected in the plasma capable of causing a slow contraction of isolated smooth muscle Paton 4 was able to demonstrate the presence of a `slow-contracting' substance in addition to histamine in the plasma of cats and dogs.
In the present study, histamine uptake into mast cells was directly dissected using a histamine-depleted mutant mast cell line, RBL-2H3 Sc Uptake-2 transporters modulate histamine uptake in mast cells, and PMAT and OCT1 may be established as candidate transporters for this uptake by studying the effect of various inhibitors.
1 The content, uptake and storage of 5‐hydroxytryptamine (5‐HT) in platelets were determined in eight manic‐depressive patients not on lithium (Li); in ten manic‐depressive patients on Li; and in ten apparently normal persons as controls.
2 Platelets from the patients, whether or not on Li, contained less 5‐HT than platelets from normal people. 3 5‐HT uptake and storage by.
The first 5-HT peak due to 48/induced mast cell release was independent of extracellular Ca/sup +2/, while the second 5-HT peak due to high K/sup +/ was not.
The uptake of 5-hydroxytryptamine, 5-hydroxytryptophan, noradrenaline and dopamine by normal mouse peritoneal mast cells was studied by means of the. Serotonin is only found in mast cells in nameable concentrations in humans in discrete pathologies such as in the stroma of carcinoid tumors or in mastocytosis (16, 83, 84).
Rodent and human mast cells express the enzyme Tph1, so that they are capable to produce serotonin and release it to form early edema in inflammation (28, 85).1.
Acta Physiol Scand. May;82(1) Uptake of 5-hydroxytryptamine by mast cell granules in vitro. Jansson SE. PMID: [PubMed - indexed for MEDLINE].5-Hydroxytryptamine (5-HT) localization has been studied in the spinal and trigeminal ganglia of adult rats using immunofluorescence, peroxidase-antiperoxidase immunocytochemistry and [3H]5-HT uptake radioautography, the latter two at the ultrastructural level.
Endoneurial mast cells, identified by alcian blue staining, formed 8 and 14% of all mast cells and neurons in spinal and trigeminal.